The phylogeny of Turdinae species (Passeriformes, Muscicapidae) remains controversial. In this study, we investigated phylogenetic relationships within this subfamily based on analysis of mitochondrial cytochrome b gene sequences. 35 species representing 16 of 20 putative Turdinae genera from Turdinae were analyzed, and Bombycilla garrulous and Bombycilla cedrorum were selected as outgroups. Altogether 983 bp gene fragments from these species were obtained, in which 399 variable sites and 349 parsimony informative sites were identified. Neighbor-joining (NJ), maximum parsimony (MP), maximum-likelihood (ML) and Bayesian (BI) analyses generated nearly identical tree topologies. In the phylogenetic trees, the species examined in the study are clustered into two clades, which are almost consistent with morphological classification. The first clade included Turdus, Zoothera and Cochoa. The second clade included Phoenicurus, Chaimarrornis, Oenanthe, Rhyacornis, Monticola, Saxicola, Erithacus, Tarsiger, Enivurus, Luscinia, Myiophoneus, Copsychus and Cercotrichas. Genus Zoothera was not a monophyletic taxon and genus Phoenicurus was paraphyletic forming a well supported clade with the addition of two monotypic genera (Rhyacornis and Chaimarrornis). The genus Monticola was sister to Saxicola first, and then clustered with Oenanthe, which indicates that Monticola is more closely related to the chat than the thrush. With regard to some genera such as Erithacus, Tarsiger, Enivurus, Luscinia, Myiophoneus, Copsychus and Cercotrichas in the second clade , phylogenetic relationships are still uncertain [Acta Zoologica Sinica 52(1): 87–98, 2006].